Areas of Focus
- Mechanistic studies and development of novel agonists and antagonists for peptide hormones and GPCR interactions
- Screening and identification of orphan peptide receptors from G protein-coupled receptors
- Development of new methods for studying peptide hormone and receptor interactions
- Screening, identification, and utilization of novel protein ligases in plants
Work Experience
- 2001-2003 - Chinese Academy of Sciences, Institute of Biochemistry and Cell Biology: Assistant Researcher, Associate Researcher
- 2003-2006 - Dartmouth College, School of Medicine: Postdoctoral Research
- 2006-Present - Tongji University, Institute of Protein Research: Researcher
Academic Background & Achievements
- 1996 - Bachelor's Degree: Jilin University, Molecular Biology
- 2001 - PhD: Shanghai Institute of Biochemistry, Chinese Academy of Sciences, Biochemistry and Molecular Biology
- Published over 70 SCI papers, total impact factor over 200, cited over 1400 times
- Led 6 National Natural Science Foundation projects, 1 major national new drug creation project, and 1 national key basic research development plan project
Publications
- An efficient peptide ligase engineered from a bamboo asparaginyl endopeptidase, Wang XB, Zhang CH, Zhang T, Li HZ, Liu YL, Xu ZG, Lei G, Cai CJ*, Guo ZY*, 2024
- Is myeloid-derived growth factor a ligand of the sphingosine-1-phosphate receptor 2?, Zheng YS, Liu YL, Xu ZG, He C*, Guo ZY*, 2024
- FAM237A, rather than peptide PEN and proCCK56-63, binds to and activates the orphan receptor GPR83, Li HZ, Wang YF, Shao XX, Liu YL, Xu ZG, Wang SL, Guo ZY*, 2023
- LEAP2 is a more conserved ligand than ghrelin for fish GHSRs, Li HZ, Shao XX, Wang YF, Liu YL, Xu ZG, Guo ZY*, 2023
- The ghrelin receptor GHSR has two efficient agonists in the lobe-finned fish Latimeria chalumnae, Li HZ, Wang YF, Zheng YS, Liu YL, Xu ZG, Guo ZY*, 2023
- Nanomolar range of FAM237B can activate receptor GPR83, Li HZ, Wang YF, Hu WF, Liu YL, Xu ZG, Guo ZY*, 2023
- Development of a general bioluminescent activity assay for peptide ligases, Zhang CH, Shao XX, Wang XB, Shou LL, Liu YL, Xu ZG, Guo ZY*, 2022
- Development of Esterase-Resistant and Highly Active Ghrelin Analogs via Thiol-Ene Click Chemistry, Li HZ, Shao XX, Shou LL, Li N, Liu YL, Xu ZG, Guo ZY*, 2022
- LEAP2 has antagonized the ghrelin receptor GHSR1a since its emergence in ancient fish, Li HZ, Shou LL, Shao XX, Li N, Liu YL, Xu ZG, Guo ZY*, 2021
- Unusual orthologs shed new light on the binding mechanism of ghrelin to its receptor GHSR1a, Li HZ, Shao XX, Shou LL, Li N, Liu YL, Xu ZG, Guo ZY*, 2021
- Identifying key residues and key interactions for the binding of LEAP2 to receptor GHSR1a, Li HZ, Li N, Shao XX, Liu YL, Xu ZG, Guo ZY*, 2020
- Hydrophobic interactions of relaxin family peptide receptor 3 with ligands identified using a NanoBiT-based binding assay, Li HZ, Li N, Shao XX, Liu YL, Xu ZG, Guo ZY*, 2020
- Identifying the binding mechanism of LEAP2 to receptor GHSR1a, Wang JH, Li HZ, Shao XX, Nie WH, Liu YL, Xu ZG, Guo ZY*, 2019
- Exploring electrostatic interactions of relaxin family peptide receptor 3 and 4 with ligands using a NanoBiT-based binding assay, Wang JH, Nie WH, Shao XX, Li HZ, Hu MJ, Liu YL, Xu ZG, Guo ZY*, 2019
- Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5, Wang JH, Shao XX, Hu MJ, Liu YL, Xu ZG, Guo ZY*, 2019
- Exploring receptor selectivity of the chimeric relaxin family peptide R3/I5 by incorporating unnatural amino acids, Wang JH, Hu MJ, Zhang L, Shao XX, Lv CH, Liu YL, Xu ZG, Guo ZY*, 2018
- Development of a novel ligand binding assay for relaxin family peptide receptor 3 and 4 using NanoLuc complementation, Hu MJ, Shao XX, Li HZ, Nie WH, Wang JH, Liu YL, Xu ZG, Guo ZY*, 2018
- Cholesterol modulates the binding properties of human relaxin family peptide receptor 3 with its ligands, Wang JH, Hu MJ, Shao XX, Wei D, Liu YL, Xu ZG, Guo ZY*, 2018
- Overexpression of relaxin family peptide receptor 3 in Escherichia coli and characterization of its ligand binding properties, Hu MJ, Wang JH, Shao XX, Liu YL, Xu ZG, Guo ZY*, 2018
- Rapid preparation of bioluminescent tracers for relaxin family peptides using sortase-catalysed ligation, Hu MJ, Wei D, Shao XX, Wang JH, Liu YL, Xu ZG, Guo ZY*, 2017
- Interaction mechanism of insulin-like peptide 5 with relaxin family peptide receptor 4, Hu MJ, Wei D, Shao XX, Wang JH, Liu YL, Xu ZG, Guo ZY*, 2017
- A novel BRET-based binding assay for interaction studies of relaxin family peptide receptor 3 with its ligands, Wang JH, Shao XX, Hu MJ, Wei D, Liu YL, Xu ZG, Guo ZY*, 2017
- Development of a selective agonist for relaxin family peptide receptor 3, Wei D, Hu MJ, Shao XX, Wang JH, Nie WH, Liu YL, Xu ZG, Guo ZY*, 2017
- A negatively charged transmembrane aspartate residue controls activation of the relaxin-3 receptor RXFP3, Liu Y, Zhang L, Shao XX, Hu MJ, Liu YL, Xu ZG, Guo ZY*, 2016
- Novel Bioluminescent Binding Assays for Ligand–Receptor Interaction Studies of the Fibroblast Growth Factor Family, Song G, Shao XX, Wu QP, Xu ZG, Liu YL*, Guo ZY*, 2016
- Mechanism for insulin-like peptide 5 distinguishing the homologous relaxin family peptide receptor 3 and 4, Hu MJ, Shao XX, Wang JH, Wei D, Guo YQ, Liu YL, Xu ZG, Guo ZY*, 2016
- Identification of hydrophobic interactions between relaxin-3 and its receptor RXFP3: implication for a conformational change in the B-chain C-terminus during receptor binding, Hu MJ, Shao XX, Wang JH, Wei D, Liu YL, Xu ZG, Guo ZY*, 2016
- Novel bioluminescent binding assays for interaction studies of protein peptide hormones with their receptors, Liu YL, Guo ZY*, 2016
- Application of the novel bioluminescent ligand–receptor binding assay to relaxin-RXFP1 system for interaction studies, Wu QP, Zhang L, Shao XX, Wang JH, Gao Y, Xu ZG, Liu YL*, Guo ZY*, 2016
- Quick preparation of nanoluciferase-based tracers for novel bioluminescent receptor-binding assays of protein hormones: using erythropoietin as a model, Song G, Wu QP, Xu T, Liu YL, Xu ZG, Zhang SF, Guo ZY*, 2015
- Novel bioluminescent receptor-binding assays for peptide hormones: using ghrelin as a model, Liu Y, Shao XX, Zhang L, Song G, Liu YL, Xu ZG, Guo ZY*, 2015
- Efficient overexpression of human interleukin-6 in Escherichia coli using nanoluciferase as a fusion partner, Ji BJ, Song G, Zhang Z, Guo ZY*
