Areas of Focus
- Development of novel single-molecule fluorescence techniques
- Study of protein targeting mechanisms on DNA
- Research on targeting and cleavage mechanisms of CRISPR-related proteins
Work Experience
- 2008-2015 - University of Pennsylvania - Postdoctoral Researcher
- 2015-2018 - Tsinghua University, School of Life Sciences - Assistant Professor
- 2018-present - Tsinghua University, School of Life Sciences - Associate Professor
Academic Background & Achievements
- 1999-2003 Bachelor: Peking University, School of Chemistry and Molecular Engineering
- 2003-2008 PhD: Peking University, School of Chemistry and Molecular Engineering
Publications
- General strategy to improve the photon budget of thiol-conjugated cyanine dyes, Chunlai Chen et al., 2023
- Nonspecific interactions between Cas12a and dsDNA located downstream of the PAM mediate target search and assist AsCas12a for DNA cleavage, Chunlai Chen et al., 2023
- Structural and dynamic mechanisms for coupled folding and tRNA recognition of a translational T-box riboswitch, Chunlai Chen et al., 2023
- Function and dynamics of the intrinsically disordered carboxyl terminus of β2 adrenergic receptor, Chunlai Chen et al., 2023
- Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination, Chunlai Chen et al., 2021
- Pseudoknot length modulates the folding, conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs, Chunlai Chen et al., 2021
- Nonspecific interactions between SpCas9 and dsDNA sites located downstream of the PAM mediate facilitated diffusion to accelerate target search, Chunlai Chen et al., 2021
- Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction, Chunlai Chen et al., 2021
- Phase separation at the nanoscale quantified by dcFCCS, Chunlai Chen et al., 2020
- Target search and recognition mechanisms of glycosylase AlkD revealed by scanning FRET-FCS and Markov State Models, Chunlai Chen et al., 2020
