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王成坤
Basic Medical Sciences
南京医科大学
江苏
Language: 英语, 中文
Crispr
基因编辑
神经精神疾病
药物靶点
分子机制
单细胞测序
Ipsc分化
谱系追踪
基因组敲入
脱靶效应
Areas of Focus
- 基因编辑系统优化
- 神经精神疾病分子机制研究及药物靶点发现
Work Experience
- 2023-至今 - 南京医科大学 - 教授
- 2018-2023 - 斯坦福大学 - 博士后研究员
Academic Background & Achievements
- 2013-2018 博士学位：浙江大学
- 在著名国际期刊上发表了20多篇SCI论文
Publications
- dCas9-based gene editing for cleavage-free genomic knock-in of long sequences, Chengkun Wang; Yuanhao Qu; Jason K. W. Cheng; Nicholas W. Hughes; Qianhe Zhang; Mengdi Wang; Le Cong, 2022
- Microbial single-strand annealing proteins enable CRISPR gene-editing tools with improved knock-in efficiencies and reduced off-target effects, Chengkun Wang; Jason K W Cheng; Qianhe Zhang; Nicholas W Hughes; Qiong Xia; Monte M Winslow; Le Cong, 2021
- CRISPR-Cas12a System With Synergistic Phage Recombination Proteins for Multiplex Precision Editing in Human Cells, Chengkun Wang; Qiong Xia; Qianhe Zhang; Yuanhao Qu; Stephen Su; Jason K. W. Cheng; Nicholas W. Hughes; Le Cong, 2022
- Programmable DNA repair with CRISPRa/i enhanced homology-directed repair efficiency with a single Cas9, Lupeng Ye; Chengkun Wang; Lingjuan Hong; Ninghe Sun; Danyang Chen; Sidi Chen; Feng Han, 2018
- Melatonin ameliorates hypoglycemic stress-induced brain endothelial tight junction injury by inhibiting protein nitration of TP53-induced glycolysis and apoptosis regulator, Chengkun Wang; Muhammad Masood Ahmed; Quan Jiang; Nan-nan Lu; Chao Tan; Yin-ping Gao; Qaisar Mahmood; Dan-yang Chen; Kohji Fukunaga; Mei Li; Zhong Chen; Christopher S. Wilcox; Yingmei Lu; Zheng-hong Qin; Feng Han, 2017
- CRISPRa screening with real world evidence identifies potassium channels as neuronal entry factors and druggable targets for SARS-CoV-2, Chengkun Wang; Ravi K. Dinesh; Yuanhao Qu; Arjun Rustagi; Henry Cousins; James Zengel; Yinglong Guo; Taryn Hall; Aimee Beck; Luke Tso; Elif Tokar Erdemic; Kae Tanudtanud; Sheng Ren; Kathy Tzy-Hwa Tzeng; Aaron Wilk; Mengdi Wang; Jan Carette; Russ Altman; Catherine A. Blish; Le Cong, 2021
- A H2O2-Responsive Theranostic Probe for Endothelial Injury Imaging and Protection, Chengkun Wang; Juan Cheng; Xingguang Liang; Chao Tan; Quan Jiang; Yongzhou Hu; Yingmei Lu; Kohji Fukunaga; Feng Han; Xin Li, 2017
- Endothelium-Derived Semaphorin 3G Regulates Hippocampal Synaptic Structure and Plasticity via Neuropilin-2/PlexinA4, Chao Tan; Nannan Lu; Chengkun Wang; Danyang Chen; Ninghe Sun; Hang Lyu; Jakob Körbelin; Weixing Shi; Kohji Fukunaga; Yingmei Lu; Feng Han, 2019
- Myt1l Induced Direct Reprogramming of Pericytes into Cholinergic Neurons, Xingguang Liang; Chao Tan; Chengkun Wang; Rongrong Tao; Yujie Huang; Kuifen Ma; Kohji Fukunaga; Mingzhu Huang; Feng Han, 2018
- Calpain activation and disturbance of autophagy are induced in cortical neurons in vitro by exposure to HA/β-Ga2O3:Cr3+ nanoparticles, Yu Lei; Chengkun Wang; Quan Jiang; Xiaoyi Sun; Yongzhong Du; Yaofeng Zhu; Yingmei Lu, 2018
