Professor | 温凯 | 数字化转型顾问

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Name	温凯
Contact Information	wenkai@cau.edu.cn
Language ability	中文, 英语
Province	北京

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Name

温凯

Contact Information

wenkai@cau.edu.cn

Language ability

中文, 英语

Province

北京

University/Lab Affiliation and Title	中国农业大学
Faculty	Veterinary Medicine
Academic Background & Achievements	2008-2012 中国农业大学基础兽医学博士 2005-2008 中国农业大学基础兽医学硕士 2001-2005 西南农业大学动物医学学士
Work Experience	2016-至今中国农业大学副研究员 2014-2015 中国农业大学讲师 2012-2014 中国农业大学博士后
Awards	2015年国家技术发明奖二等奖 2021年第十二届大北农科技奖一等奖 2021年北京市科学技术进步奖二等奖 2023年第九届中国国际“互联网+”大学生创新创业大赛北京赛区一等奖

University/Lab Affiliation and Title

中国农业大学

Faculty

Veterinary Medicine

Academic Background & Achievements

2008-2012 中国农业大学基础兽医学博士
2005-2008 中国农业大学基础兽医学硕士
2001-2005 西南农业大学动物医学学士

Work Experience

2016-至今中国农业大学副研究员
2014-2015 中国农业大学讲师
2012-2014 中国农业大学博士后

Awards

2015年国家技术发明奖二等奖
2021年第十二届大北农科技奖一等奖
2021年北京市科学技术进步奖二等奖
2023年第九届中国国际“互联网+”大学生创新创业大赛北京赛区一等奖

Areas of Focus	动物源食品安全兽药残留快速检测技术兽医公共卫生学兽医药理与毒理学
Publications	Broad-specificity indirect competitive enzyme-linked immunosorbent assay for aristolochic acids: Computer-aided hapten design and molecular mechanism of antibody recognition, Wang X, Lu Q, Dou L, Liu M, Li P, Yu W, Yu X, Wang Z, Wen K, 2023 Rapid On-Site Detection of Extensively Drug-Resistant Genes in Enterobacteriaceae via Enhanced Recombinase Polymerase Amplification and Lateral Flow Biosensor, Tao J, Liu D, Xiong J, Dou L, Zhai W, Zhang R, Wang Y, Shen J, Wen K, 2022 Development of a new broad-specific monoclonal antibody with uniform affinity for aflatoxins and magnetic beads-based enzymatic immunoassay, Zhang X, Song M, Yu X, Wang Z, Ke Y, Jiang H, Li J, Shen J, Wen K, 2017 Universal simultaneous multiplex ELISA of small molecules in milk based on dual luciferases, Yu X, Zhang X, Wang Z, Jiang H, Lv Z, Shen J, Xia G, Wen K, 2018 Preparation of high affinity antibody for ribavirin with new haptens and residue analysis in chicken muscle, eggs and duck muscle, Wang Z, Yu X, Ma L, Liu H, Ding S, Wang Z, Zhang X, Shen J, Wen K, 2018 Development of low matrix effects method for the analysis of bisphenol A and bisphenol S in aquatic products by immunoaffinity purification, Shan W, Yao K, Wen K, Shao B, 2019 Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk, Ma L, Wang Z, Liu H, Wu C, Ding Y, Wen K, 2019 Development of a fluorescence immunoassay for highly sensitive detection of amantadine using the nanoassembly of carbon dots and MnO2 nanosheets as the signal probe, Dong B, Li H, Sun J, Mari GM, Yu X, Ke Y, Li J, Wang Z, Yu W, Wen K, Shen J, 2019 Influence of small molecular property on antibody response, Wen K#, Bai Y#, Wei Y, Li C, Shen J, Wang Z, 2020 Hapten Synthesis and Monoclonal Antibody Preparation for Simultaneous Detection of Albendazole and Its Metabolites in Animal-Origin Food, Shao S, Zhou X, Dou L, Bai Y, Mi J, Yu W, Zhang S, Wang Z, Wen K, 2021 Anti-Metatype Antibody Screening, Sandwich Immunoassay Development, and Structural Insights for β-Lactams Based on Penicillin Binding Protein, Bai Y, Dou L, Wu W, Lu Z, Kou J, Shen J, Wen K, Wang Z, 2021 Binding affinity-guided design of a highly sensitive noncompetitive immunoassay for small molecule detection, Bai Y, Wang Y, Li Q, Dou L, Liu M, Shao S, Zhu J, Shen J, Wang Z, Wen K, Yu W, 2021 Portable magnetofluidic device for point-of-need detection of African swine fever, Chen L#, Wen K#, Chen FE, Trick AY, Liu H, Shao S, Yu W, Hsieh K, Wang Z, Shen J, Wang TH, 2021 Synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for amantadine with high sensitivity in chicken, Guo L, Liu M, Li Q, Dong B, Li H, Mari GM, Liu R, Yu W, Yu X, Wang Z, Zhang S, Shen J, Wen K, 2021 Trace detection of bromadiolone and brodifacoum in biological and food samples: A label-free fluorescence immunoassay based on an in situ formation strategy, Chen J, Huang B, Kong L, Sun F, Li L, Shao B, Peng C, Cai H, Hou R, Wen K, Wang Z, Li H, 2023

Areas of Focus

动物源食品安全
兽药残留快速检测技术
兽医公共卫生学
兽医药理与毒理学

Publications

Broad-specificity indirect competitive enzyme-linked immunosorbent assay for aristolochic acids: Computer-aided hapten design and molecular mechanism of antibody recognition, Wang X, Lu Q, Dou L, Liu M, Li P, Yu W, Yu X, Wang Z, Wen K*, 2023
Rapid On-Site Detection of Extensively Drug-Resistant Genes in Enterobacteriaceae via Enhanced Recombinase Polymerase Amplification and Lateral Flow Biosensor, Tao J, Liu D, Xiong J, Dou L, Zhai W, Zhang R, Wang Y, Shen J*, Wen K*, 2022
Development of a new broad-specific monoclonal antibody with uniform affinity for aflatoxins and magnetic beads-based enzymatic immunoassay, Zhang X, Song M, Yu X, Wang Z, Ke Y, Jiang H, Li J, Shen J, Wen K*, 2017
Universal simultaneous multiplex ELISA of small molecules in milk based on dual luciferases, Yu X, Zhang X, Wang Z, Jiang H, Lv Z, Shen J, Xia G, Wen K*, 2018
Preparation of high affinity antibody for ribavirin with new haptens and residue analysis in chicken muscle, eggs and duck muscle, Wang Z, Yu X, Ma L, Liu H, Ding S, Wang Z, Zhang X, Shen J, Wen K*, 2018
Development of low matrix effects method for the analysis of bisphenol A and bisphenol S in aquatic products by immunoaffinity purification, Shan W, Yao K, Wen K*, Shao B*, 2019
Monoclonal antibody production and the development of a quantitative time-resolved fluoroimmunoassay for rifaximin in milk, Ma L, Wang Z, Liu H, Wu C, Ding Y, Wen K*, 2019
Development of a fluorescence immunoassay for highly sensitive detection of amantadine using the nanoassembly of carbon dots and MnO2 nanosheets as the signal probe, Dong B, Li H, Sun J, Mari GM, Yu X, Ke Y, Li J, Wang Z, Yu W*, Wen K*, Shen J, 2019
Influence of small molecular property on antibody response, Wen K#, Bai Y#, Wei Y, Li C, Shen J, Wang Z, 2020
Hapten Synthesis and Monoclonal Antibody Preparation for Simultaneous Detection of Albendazole and Its Metabolites in Animal-Origin Food, Shao S, Zhou X, Dou L, Bai Y, Mi J, Yu W, Zhang S, Wang Z, Wen K*, 2021
Anti-Metatype Antibody Screening, Sandwich Immunoassay Development, and Structural Insights for β-Lactams Based on Penicillin Binding Protein, Bai Y, Dou L, Wu W, Lu Z, Kou J, Shen J, Wen K*, Wang Z, 2021
Binding affinity-guided design of a highly sensitive noncompetitive immunoassay for small molecule detection, Bai Y, Wang Y, Li Q, Dou L, Liu M, Shao S, Zhu J, Shen J, Wang Z, Wen K*, Yu W*, 2021
Portable magnetofluidic device for point-of-need detection of African swine fever, Chen L#, Wen K#, Chen FE, Trick AY, Liu H, Shao S, Yu W, Hsieh K, Wang Z, Shen J, Wang TH, 2021
Synthesis and characterization of tracers and development of a fluorescence polarization immunoassay for amantadine with high sensitivity in chicken, Guo L, Liu M, Li Q, Dong B, Li H, Mari GM, Liu R, Yu W, Yu X, Wang Z, Zhang S, Shen J, Wen K*, 2021
Trace detection of bromadiolone and brodifacoum in biological and food samples: A label-free fluorescence immunoassay based on an in situ formation strategy, Chen J, Huang B, Kong L, Sun F, Li L, Shao B, Peng C, Cai H, Hou R, Wen K*, Wang Z, Li H*, 2023

食品安全抗体核酸疾病诊断兽药毒素非洲猪瘟抗原设计免疫分析分子生物学

江海洋 - 中国农业大学

汪铭书 - 四川农业大学

沈建忠 - 中国农业大学

Area of Focus
兽药 | 残留检测 | 动物源性食品 | 细菌耐药性 | 形成 | 控制 | 食品安全 | 抗生素 | 病原体

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